Metam
ORF

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Open Reading Frame (ORF)

ORF

ID CARD

MetamORF ID

1088

Chromosome

4

Strand

+

Start-stop positions

155553450-155553503

Nucleic length (bp)

54

Sequences

Nucleic sequence

CTG ...

Amino acid sequence

LGC ...

Spliced: No

Exons

1

Exons start-end

Transcripts

7 known transcripts

MetamORF
transcript ID

The MetamORF ID of the transcript. This is an arbitrary ID that does not correspond to any official (Ensembl, NCBI...) transcript ID or external reference.

Transcript
ID

The official transcript ID (usually an Ensembl ID, e.g. ENST00000395565).

Transcript biotype

The biotype of the transcript (as defined by Ensembl).

Gene ID

The gene ID. Usually the HGNC ID for H. sapiens genes and the NCBI ID for M. musculus genes.

Relative
positions

The relative positions of the start and stop codons of the ORF on the transcript.

Identification

The method of identification used to identify the ORF. MetamORF currently integrates data from three main type of identification methods: bioinformatic predictions, ribosome profiling experiments and mass spectrometry experiments (either proteomics or proteogenomics). See the data sources section of the advances documentation for more information about this.

Start flanking
sequence

The sequence flanking the start codon of the ORF on the transcript. This sequence registered the nucleotides from -6 to +4 positions, where +1 corresponds to the first nucleotide of the ORF start codon.

Kozak
context

The Kozak context computed by our algorithm for the ORF on the transcript. See the Kozak contexts section of the advanced documentation for more details regarding the nomenclature we use.

Exp.
count

The number of original datasets that identifed the ORF on the transcript.

Data sources

The data sources in which the ORF has been identified. Click on the button to display all the original IDs in a pop-up. See the data sources section of the advanced documentation for more details regarding the information related to the data sources and the original ORF IDs.

ORF
annotations

A comma-separated list of the annotations computed by our algorithm for the ORF on the transcript. See the section dedicated to ORF annotations in the advanced documentation for more details regarding the nomenclature we use.

Cell types

A comma-separated list of the cell types in which the ORF has already been identified on the transcript.

145517 ENSMUST00000175705 retained_intron NCBI:14688 466 - 519 Ribo-seq
CAAGCTCTGG strong 1 sORFs_org_Mouse
    Intronic
    sORF
Spleen_B_cell
146667 ENSMUST00000176637 protein_coding NCBI:14688 630 - 683 Ribo-seq
CAAGCTCTGG strong 3 sORFs_org_Mouse
    Alternative
    InCDS
    Overlapping
    sORF
Brain,
MEF
657 ENSMUST00000165335 protein_coding NCBI:14688 939 - 992 Ribo-seq
CAAGCTCTGG strong 1 sORFs_org_Mouse
    Alternative
    InCDS
    Overlapping
    sORF
Skin_tumor
1058383 ENSMUST00000176308 retained_intron NCBI:14688 227 - 280 Ribo-seq
CAAGCTCTGG strong 4 sORFs_org_Mouse
    Intronic
    sORF
E14,
MEF, NSC, v6-5
515292 ENSMUST00000177094 protein_coding NCBI:14688 867 - 920 Ribo-seq
CAAGCTCTGG strong 1 sORFs_org_Mouse
    sORF
R1E
55620 ENSMUST00000105616 protein_coding NCBI:14688 887 - 940 Ribo-seq
CAAGCTCTGG strong 2 sORFs_org_Mouse
    Alternative
    InCDS
    Overlapping
    sORF
Liver,
MEF
59781 ENSMUST00000030940 protein_coding NCBI:14688 930 - 983 Ribo-seq
CAAGCTCTGG strong 3 sORFs_org_Mouse
    Alternative
    InCDS
    Overlapping
    sORF
BMDC,
MEF

Export data

Identification method

Predicted

Ribo-seq

MS

Kozak context

moderate

weak

strong

optimal

Transcript biotype

antisense

antisense_RNA

bidirectional_promoter_lncRNA

IG_C_gene

lincRNA

non_stop_decay

nonsense_mediated_decay

polymorphic_pseudogene

processed_pseudogene

processed_transcript

protein_coding

pseudogene

retained_intron

sense_intronic

sense_overlapping

TEC

transcribed_processed_pseudogene

transcribed_unitary_pseudogene

transcribed_unprocessed_pseudogene

unprocessed_pseudogene

ORF Annotations

Reading frame

Alternative

Relative position

CDS

Downstream

InCDS

Intronic

NewCDS

Overlapping

Upstream

Biotype

Intergenic

ncRNA

NMD

NSD

Pseudogene

Length

sORF

Cell types

3T3

B_cell

BMDC

Brain

C2C12

E14

Glioma

Liver

MEF

MESC

Neutrophil

NSC

R1E

Skin_tumor

Spleen_B_cell

Testis

v6-5